This study deals with the conversion of organic matter into methane at ambient temperature, during anaerobic digestion of domestic wastewater combined with a submerged ultrafiltration membrane with no gas-sparging. A one-stage submerged granular anaerobic membrane bioreactor (G-AnMBR) and a control anaerobic digester (UASB type) were operated during four months, after 500 days of biomass acclimatization to psychrophilic and low loading rate conditions. Membrane barrier led to the retention of biomass, suspended solids and dissolved and colloidal organic matter which greatly enhanced total COD (tCOD) removal (92.3%) and COD to methane conversion (84.7% of tCOD converted into dissolved and gaseous CH4). G-AnMBR overcame the usual long start-up period and led to a higher sludge heterogeneity, without altering the granular biomass activity. The feasibility of the G-AnMBR without gas-sparging was also assessed and the net positive energy balance was estimated around + 0.58 kWh.m−3.