Potential genetic robustness of Prnp and Sprn double knockout mouse embryos towards ShRNA-lentiviral inoculation - Archive ouverte HAL Access content directly
Journal Articles Veterinary Research Year : 2022

Potential genetic robustness of Prnp and Sprn double knockout mouse embryos towards ShRNA-lentiviral inoculation

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Abstract

The Shadoo and PrP prion protein family members are thought to be functionally related, but previous knockdown/ knockout experiments in early mouse embryogenesis have provided seemingly contradictory results. In particular, Shadoo was found to be indispensable in the absence of PrP in knockdown analyses, but a double-knockout of the two had little phenotypic impact. We investigated this apparent discrepancy by comparing transcriptomes of WT, Prnp 0/0 and Prnp 0/0 Sprn 0/0 E6.5 mouse embryos following inoculation by Sprn-or Prnp-ShRNA lentiviral vectors. Our results suggest the possibility of genetic adaptation in Prnp 0/0 Sprn 0/0 mice, thus providing a potential explanation for their previously observed resilience.
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Dates and versions

hal-03718138 , version 1 (08-07-2022)

Licence

Attribution - CC BY 4.0

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Andrea Rau, Bruno Passet, Johan Castille, Nathalie Daniel-Carlier, Alexandre Asset, et al.. Potential genetic robustness of Prnp and Sprn double knockout mouse embryos towards ShRNA-lentiviral inoculation. Veterinary Research, 2022, 53, ⟨10.1186/s13567-022-01075-4⟩. ⟨hal-03718138⟩
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