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Journal Articles Nucleic Acids Research Year : 2021

Conservation and divergence of meiotic DNA double strand break forming mechanisms in Arabidopsis thaliana

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Abstract

Abstract In the current meiotic recombination initiation model, the SPO11 catalytic subunits associate with MTOPVIB to form a Topoisomerase VI-like complex that generates DNA double strand breaks (DSBs). Four additional proteins, PRD1/AtMEI1, PRD2/AtMEI4, PRD3/AtMER2 and the plant specific DFO are required for meiotic DSB formation. Here we show that (i) MTOPVIB and PRD1 provide the link between the catalytic sub-complex and the other DSB proteins, (ii) PRD3/AtMER2, while localized to the axis, does not assemble a canonical pre-DSB complex but establishes a direct link between the DSB-forming and resection machineries, (iii) DFO controls MTOPVIB foci formation and is part of a divergent RMM-like complex including PHS1/AtREC114 and PRD2/AtMEI4 but not PRD3/AtMER2, (iv) PHS1/AtREC114 is absolutely unnecessary for DSB formation despite having a conserved position within the DSB protein network and (v) MTOPVIB and PRD2/AtMEI4 interact directly with chromosome axis proteins to anchor the meiotic DSB machinery to the axis.

Dates and versions

hal-03744283 , version 1 (02-08-2022)

Licence

Attribution - CC BY 4.0

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Nathalie Vrielynck, Katja Schneider, Marion Rodriguez, Jason Sims, Aurélie Chambon, et al.. Conservation and divergence of meiotic DNA double strand break forming mechanisms in Arabidopsis thaliana. Nucleic Acids Research, 2021, 49 (17), pp.9821-9835. ⟨10.1093/nar/gkab715⟩. ⟨hal-03744283⟩
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