In this study we examine Cu content and isotopic ratios in soils, soil solutions and organs (i.e., roots and leaves) of grapevine plants (i.e., Vitis vinifera – cv. Tanat) grown in pot experiments in greenhouse. Six different soils from three wine-growing areas (i.e., Pessac-Leognan, France; Saint Mont, France and Soave, Italy) were selected for their distinct Cu pesticide input history and their pedological characteristics. We calculated Cu-isotope fractionation between soil and soil solution, and during Cu uptake and transfer within the plants (i.e., from soil solution to roots and from roots to leaves) in order to better understand the effect of plant homeostasis on Cu behavior and isotope signature in the soil-plant system.