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Journal Articles Molecular Cell Year : 2003

A Two-Protein Strategy for the Functional Loading of a Cellular Replicative DNA Helicase

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Abstract

The delivery of a ring-shaped hexameric helicase onto DNA is a fundamental step of DNA replication, conserved in all cellular organisms. We report the biochemical characterization of the bacterial hexameric replicative helicase DnaC of Bacillus subtilis with that of the two replication initiation proteins DnaI and DnaB. We show that DnaI and DnaB interact physically and functionally with the DnaC helicase and mediate its functional delivery onto DNA. Thus, DnaB and DnaI form a pair of helicase loaders, revealing a two-protein strategy for the loading of a replicative helicase. We also present evidence that the DnaC helicase loading mechanism appears to be of the ring-assembly type, proceeding through the recruitment of DnaC monomers and their hexamerization around single-stranded DNA by the coordinated action of DnaI and DnaB.

Dates and versions

hal-03760949 , version 1 (25-08-2022)

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Marion Velten, Stephen Mcgovern, Stéphanie Marsin, S.Dusko Ehrlich, Philippe P. Noirot, et al.. A Two-Protein Strategy for the Functional Loading of a Cellular Replicative DNA Helicase. Molecular Cell, 2003, 11 (4), pp. 1009-1020. ⟨10.1016/S1097-2765(03)00130-8⟩. ⟨hal-03760949⟩
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