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Metabolomic and photosynthesis responses of freshwater periphyton to natural vs synthetic fungicides

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Abstract

Agricultural activity leads to the release of thousands of compounds such as fungicides to aquatic ecosystems that might be harmful for exposed organisms such as periphytic microbial communities that play key role in ecosystem functions. Although previous studies have shown adverse effect of fungicides on the structure and functions of periphytic biofilms or their component, there is still a paucity of knowledge on short term molecular/biochemical mechanisms involved in such long term impairment on ecosystem function(s). This is strongly needed to support the discovery of biomarkers allowing the early and sensitive detection of the alteration of ecosystem function. To tackle this challenge, untargeted meta-metabolomics is an approach of choice since it provides a comprehensive picture of the biochemical activity and the phenotype (i.e. molecular phenotype through the identification of metabolites and their pathways) of a community, as a result of interactions with the environment. In this context, the present study aims to highlight the link between short-term response at the molecular level (metabolomics) and long term response at the function level (photosynthesis, microbial activity)of periphytic biofilms to the model fungicides copper (Cu, as natural fungicide) and tebuconazole (TBZ, as synthetic fungicide). To this end, short-term exposure (4h) to serial dilution of Cu and TBZ were first performed under controlled conditions (T°C 18°C, L/d 12:12). Following these exposure, photosynthetic yield using Phyto-PAM (as function) and metabolomics analysis were implemented. The results showed effect of copper on both photosynthetic activity and metabolic pattern whereas only metabolomics responded to tebuconazole. According this first experiment, two concentrations have been selected in order to further implement one month exposure to determine the kinetic of responses and their synchronism. This investigation will provide additional evidence about long-term effect on microbial activity.
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Dates and versions

hal-03799950 , version 1 (06-10-2022)

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  • HAL Id : hal-03799950 , version 1

Cite

Ilan Viemont-Lefeuvre, Mélissa Eon, Arthur Médina, Nicolas Creusot. Metabolomic and photosynthesis responses of freshwater periphyton to natural vs synthetic fungicides. 7th Biofilm Workshop, Jun 2022, Bordeaux, France. ⟨hal-03799950⟩

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