Feeding of palm oil fatty acids or rapeseed oil throughout lactation: Effects on mammary gene expression and milk production in Norwegian dairy goats
Résumé
Lipid added as rapeseed or palm oil to the diet of
dairy goats over 8 mo of one lactation alters fat secre-
tion and milk fatty acid (FA) and protein composition.
In this study, we examined the contribution of mam-
mary gene expression to these changes and included 30
multiparous goats of Norwegian dairy goat breed for a
230-d experimental period, with indoor feeding from 1
to 120 d in milk (DIM), mountain grazing from 120 to
200 DIM, and indoor feeding from 200 to 230 DIM. Af-
ter an initial period (1–60 DIM) when the control diet
was given to all goats, the animals were subdivided into
3 groups of 10 goats. Treatments (60–230 DIM) were
basal concentrate (control) alone or supplemented with
either 8% (by weight) hydrogenated palm oil enriched
with palmitic acid (POFA) or 8% (by weight) rapeseed
oil (RSO). Milk was sampled individually from all ani-
mals throughout lactation, at 60, 120, 190, and 230 DIM
for milk yield and composition. On d 60, 120, 190, and
230, mammary tissue was collected by biopsy to mea-
sure mRNA abundance of 19 key genes. None of the 19
genes involved in milk protein, apoptosis, lipid metabo-
lism, transcription factors, and protein of the milk fat
globule membrane, as measured by mRNA abundance,
were affected by the lipid supplements, although POFA
increased milk fat content, and POFA and RSO affect-
ed milk FA composition. Over the experimental period
(120–230 DIM), the mRNA abundance of 13 of the 19
studied genes was affected by lactation stage. For some
genes, expression either gradually increased from 120 to
230 DIM (CSN2, CASP8, CD36, GLUT4) or increased
from 120 to 200 and then remained stable (XDH), or
decreased (CSN3, G6PD, SREBF1, PPARG1) or in-
creased only at 230 DIM (SCD1, SCD5, ELF3). For a
second group of genes (CSN1, LALBA, FABP3, FASN,
LPL, MFGE8), expression was stable over the lactationexpression, such as substrate availability or posttran-
scriptional regulation of these genes, could play an im-
portant role in the milk fat and FA responses to dietary
fat composition in the goat. In conclusion, mammary
gene expression in goats was more regulated by stage of
lactation than by the dietary treatments applied.
period. Our results suggest that factors other than gene