Feeding of palm oil fatty acids or rapeseed oil throughout lactation: Effects on mammary gene expression and milk production in Norwegian dairy goats - Archive ouverte HAL Access content directly
Journal Articles Journal of Dairy Science Year : 2022

Feeding of palm oil fatty acids or rapeseed oil throughout lactation: Effects on mammary gene expression and milk production in Norwegian dairy goats

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K. Hove
H. Volden
R.A. Inglingstad
M. Eknæs

Abstract

Lipid added as rapeseed or palm oil to the diet of dairy goats over 8 mo of one lactation alters fat secre- tion and milk fatty acid (FA) and protein composition. In this study, we examined the contribution of mam- mary gene expression to these changes and included 30 multiparous goats of Norwegian dairy goat breed for a 230-d experimental period, with indoor feeding from 1 to 120 d in milk (DIM), mountain grazing from 120 to 200 DIM, and indoor feeding from 200 to 230 DIM. Af- ter an initial period (1–60 DIM) when the control diet was given to all goats, the animals were subdivided into 3 groups of 10 goats. Treatments (60–230 DIM) were basal concentrate (control) alone or supplemented with either 8% (by weight) hydrogenated palm oil enriched with palmitic acid (POFA) or 8% (by weight) rapeseed oil (RSO). Milk was sampled individually from all ani- mals throughout lactation, at 60, 120, 190, and 230 DIM for milk yield and composition. On d 60, 120, 190, and 230, mammary tissue was collected by biopsy to mea- sure mRNA abundance of 19 key genes. None of the 19 genes involved in milk protein, apoptosis, lipid metabo- lism, transcription factors, and protein of the milk fat globule membrane, as measured by mRNA abundance, were affected by the lipid supplements, although POFA increased milk fat content, and POFA and RSO affect- ed milk FA composition. Over the experimental period (120–230 DIM), the mRNA abundance of 13 of the 19 studied genes was affected by lactation stage. For some genes, expression either gradually increased from 120 to 230 DIM (CSN2, CASP8, CD36, GLUT4) or increased from 120 to 200 and then remained stable (XDH), or decreased (CSN3, G6PD, SREBF1, PPARG1) or in- creased only at 230 DIM (SCD1, SCD5, ELF3). For a second group of genes (CSN1, LALBA, FABP3, FASN, LPL, MFGE8), expression was stable over the lactationexpression, such as substrate availability or posttran- scriptional regulation of these genes, could play an im- portant role in the milk fat and FA responses to dietary fat composition in the goat. In conclusion, mammary gene expression in goats was more regulated by stage of lactation than by the dietary treatments applied. period. Our results suggest that factors other than gene
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Dates and versions

hal-03811690 , version 1 (12-10-2022)

Licence

Attribution - CC BY 4.0

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Cite

L. Bernard, Y. Chilliard, K. Hove, H. Volden, R.A. Inglingstad, et al.. Feeding of palm oil fatty acids or rapeseed oil throughout lactation: Effects on mammary gene expression and milk production in Norwegian dairy goats. Journal of Dairy Science, 2022, Sous Presse, ⟨10.3168/jds.2021-21372⟩. ⟨hal-03811690⟩

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