A five-transgene cassette confers broad-spectrum resistance to a fungal rust pathogen in wheat
Ming Luo
(1)
,
Liqiong Xie
(2)
,
Soma Chakraborty
(1)
,
Aihua Wang
(3, 4)
,
Oadi Matny
(5)
,
Michelle Jugovich
(5)
,
James Kolmer
(6)
,
Terese Richardson
(1)
,
Dhara Bhatt
(1)
,
Mohammad Hoque
(1)
,
Mehran Patpour
(7)
,
Chris Sørensen
(7)
,
Diana Ortiz
(8, 9)
,
Peter Dodds
(10)
,
Burkhard Steuernagel
(11)
,
Brande Wulff
(12)
,
Narayana Upadhyaya
(8)
,
Rohit Mago
(8)
,
Sambasivam Periyannan
(8)
,
Evans Lagudah
(13, 8)
,
Roger Freedman
,
T. Lynne Reuber
,
Brian Steffenson
,
Michael Ayliffe
(8)
1
CSIRO Entomology
2 Xinjiang University
3 College of Life Science
4 Hebei Normal University
5 UMN - University of Minnesota System
6 UMN - University of Minnesota [Twin Cities]
7 Aarhus University [Aarhus]
8 CSIRO - Commonwealth Scientific and Industrial Research Organisation [Canberra]
9 GAFL - Génétique et Amélioration des Fruits et Légumes
10 CSIRO - CSIRO Agriculture and Food
11 TSL - The Sainsbury Laboratory [Norwich]
12 John Innes Centre [Norwich]
13 Plant Industry
2 Xinjiang University
3 College of Life Science
4 Hebei Normal University
5 UMN - University of Minnesota System
6 UMN - University of Minnesota [Twin Cities]
7 Aarhus University [Aarhus]
8 CSIRO - Commonwealth Scientific and Industrial Research Organisation [Canberra]
9 GAFL - Génétique et Amélioration des Fruits et Légumes
10 CSIRO - CSIRO Agriculture and Food
11 TSL - The Sainsbury Laboratory [Norwich]
12 John Innes Centre [Norwich]
13 Plant Industry
Oadi Matny
- Fonction : Auteur
- PersonId : 1178634
- ORCID : 0000-0002-8447-2886
Mehran Patpour
- Fonction : Auteur
- PersonId : 1178635
- ORCID : 0000-0002-4914-2697
Chris Sørensen
- Fonction : Auteur
- PersonId : 1178636
- ORCID : 0000-0002-3025-5843
Peter Dodds
- Fonction : Auteur
- PersonId : 1178637
- ORCID : 0000-0003-0620-5923
Burkhard Steuernagel
- Fonction : Auteur
- PersonId : 1178638
- ORCID : 0000-0002-8284-7728
Brande Wulff
- Fonction : Auteur
- PersonId : 1178639
- ORCID : 0000-0003-4044-4346
Narayana Upadhyaya
- Fonction : Auteur
- PersonId : 800947
- ORCID : 0000-0002-3052-0416
- IdRef : 115122028
Rohit Mago
- Fonction : Auteur
- PersonId : 800946
- ORCID : 0000-0001-5286-073X
Evans Lagudah
- Fonction : Auteur
- PersonId : 1178640
- ORCID : 0000-0002-6234-1789
Roger Freedman
- Fonction : Auteur
T. Lynne Reuber
- Fonction : Auteur
Brian Steffenson
- Fonction : Auteur
- PersonId : 1178641
- ORCID : 0000-0001-7961-5363
Michael Ayliffe
- Fonction : Auteur
- PersonId : 1178642
- ORCID : 0000-0003-3815-7524
Résumé
Combining fungal-resistance genes into a single cassette enables the generation of highly resistant wheat lines.
Breeding wheat with durable resistance to the fungal pathogen Puccinia graminis f. sp. tritici (Pgt), a major threat to cereal production, is challenging due to the rapid evolution of pathogen virulence. Increased durability and broad-spectrum resistance can be achieved by introducing more than one resistance gene, but combining numerous unlinked genes by breeding is laborious. Here we generate polygenic Pgt resistance by introducing a transgene cassette of five resistance genes into bread wheat as a single locus and show that at least four of the five genes are functional. These wheat lines are resistant to aggressive and highly virulent Pgt isolates from around the world and show very high levels of resistance in the field. The simple monogenic inheritance of this multigene locus greatly simplifies its use in breeding. However, a new Pgt isolate with virulence to several genes at this locus suggests gene stacks will need strategic deployment to maintain their effectiveness.