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Journal Articles Nature Plants Year : 2021

Genomics accelerated isolation of a new stem rust avirulence gene–wheat resistance gene pair

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Vinay Panwar
  • Function : Author
Ming Luo
  • Function : Author
Jian Chen
Hoa Nguyen-Phuc
Dhara Bhatt
  • Function : Author
Kostya Kanyuka
Peter Dodds

Abstract

Stem rust caused by the fungus Puccinia graminis f. sp. tritici (Pgt) is a devastating disease of the global staple crop wheat. Although this disease was largely controlled in the latter half of the twentieth century, new virulent strains of Pgt, such as Ug99, have recently evolved(1,2). These strains have caused notable losses worldwide and their continued spread threatens global wheat production. Breeding for disease resistance provides the most cost-effective control of wheat rust diseases(3). A number of rust resistance genes have been characterized in wheat and most encode immune receptors of the nucleotide-binding leucine-rich repeat (NLR) class(4), which recognize pathogen effector proteins known as avirulence (Avr) proteins(5). However, only two Avr genes have been identified in Pgt so far, AvrSr35 and AvrSr50 (refs. (6,7)), and none in other cereal rusts(8,9). The Sr27 resistance gene was first identified in a wheat line carrying an introgression of the 3R chromosome from Imperial rye(10). Although not deployed widely in wheat, Sr27 is widespread in the artificial crop species Triticosecale (triticale), which is a wheat-rye hybrid and is a host for Pgt(11,12). Sr27 is effective against Ug99 (ref. (13)) and other recent Pgt strains(14,15). Here, we identify both the Sr27 gene in wheat and the corresponding AvrSr27 gene in Pgt and show that virulence to Sr27 can arise experimentally and in the field through deletion mutations, copy number variation and expression level polymorphisms at the AvrSr27 locus. New emerged variants of stem rust threaten wheat production globally. Here, mutational genomics is used to characterize a new resistance gene named Sr27 both in wheat and in triticale, and the corresponding secreted effector in the pathogen, AvrSr27.

Dates and versions

hal-03828528 , version 1 (25-10-2022)

Identifiers

Cite

Narayana Upadhyaya, Rohit Mago, Vinay Panwar, Tim Hewitt, Ming Luo, et al.. Genomics accelerated isolation of a new stem rust avirulence gene–wheat resistance gene pair. Nature Plants, 2021, 7 (9), pp.1220-1228. ⟨10.1038/s41477-021-00971-5⟩. ⟨hal-03828528⟩
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