CD163-Expressing Porcine Macrophages Support NADC30-like and NADC34-like PRRSV Infections - Archive ouverte HAL Access content directly
Journal Articles Viruses Year : 2022

CD163-Expressing Porcine Macrophages Support NADC30-like and NADC34-like PRRSV Infections

(1, 2, 3, 4) , (1, 2, 3, 4) , (1, 2, 3, 4) , (1, 2, 3, 4) , (1, 2, 3, 4) , (1, 2, 3, 4) , (1, 2, 3, 4) , (5, 6) , (1, 2, 3, 4) , (1, 2, 3, 4)
1
2
3
4
5
6

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) has a strict cell tropism. In addition to the primary alveolar macrophages, PRRSV is strictly cytotropic to African green monkey kidney cells, such as MARC-145 cells; however, MARC-145 cells are not infected by most NADC30-like and NADC34-like PRRSV strains. The essential scavenger receptor CD163 has been proved to mediate productive infection of PRRSV in various non-permissive cell lines. In this study, we systematically tested the porcine CD163 stably expressing 3D4/21 cells for infections with various PRRSV strains. The results showed that the porcine CD163-expressing macrophages support the infections of PRRSV2 of lineages 1, 5, and 8, as evidenced by Western blotting, immunofluorescence assay, quantitative PCR, and virus titration assay. Considering the current prevalence of NADC30-like and NADC34-like PRRSV2 of lineage 1 in China, the CD163-expressing macrophages are very useful for PRRSV research and disease management.

Dates and versions

hal-03832261 , version 1 (27-10-2022)

Licence

Attribution - CC BY 4.0

Identifiers

Cite

Yulin Xu, Mengxue Ye, Shaohua Sun, Qi Cao, Jia Luo, et al.. CD163-Expressing Porcine Macrophages Support NADC30-like and NADC34-like PRRSV Infections. Viruses, 2022, 14 (9), pp.2056. ⟨10.3390/v14092056⟩. ⟨hal-03832261⟩

Collections

INRA UNAM INRAE
0 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More