Monitoring systemic infection by cucumber mosaic virus using a small fluorescent protein iLOV in plants - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Journal of General Plant Pathology Year : 2023

Monitoring systemic infection by cucumber mosaic virus using a small fluorescent protein iLOV in plants

Abstract

Tracking virus infection in plants is useful to assess plant susceptibility or resistance. The recombinant cucumber mosaic virus (CMV) expressing a 2b-fused green fluorescent protein (GFP, 25 kDa) gradually loses (within weeks) the GFP gene in plants. Here, we constructed CMV expressing a flavin-based small fluorescent protein (iLOV, 10 kDa). CMV-iLOV still retains iLOV after 28 dpi in upper leaves of inoculated Nicotiana benthamiana and allows long-term monitoring of its distribution in plants. Using CMV-iLOV, we showed that pot1, a recessive allele for resistance to potyviruses, does not confer CMV resistance in tomato.

Dates and versions

hal-03842682 , version 1 (07-11-2022)

Licence

Identifiers

Cite

Ayaka Kawakubo, Jean-Luc Gallois, Kenji Nakahara. Monitoring systemic infection by cucumber mosaic virus using a small fluorescent protein iLOV in plants. Journal of General Plant Pathology, 2023, 89, pp.47-52. ⟨10.1007/s10327-022-01100-6⟩. ⟨hal-03842682⟩
21 View
0 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More