Growing vine without chemical pesticides is a big challenge for this emblematic crop. Eliminating pesticides requires multiple management solutions – biological regulation, plant immunity stimulation, genetic resistance, for example – each of which yields only partial effects. The goal is to move to an agroecological approach based on prophylaxis, monitoring and better resilience of winegrowing systems. These control methods must be integrated into new protection strategies that maximize their combined effects while adapting them to local environmental factors, socio-economical contexts, and market issues. VITAE is an interdisciplinary project that adopt a pesticide-free paradigm to address economic and technological conditions that will favor the transition in winegrowing systems. Founded by the National Research Agency (3 M€, 2021-2026), research fronts addressed in Vitae are related to four strategies: (1) mobilizing microbiota and diversifying biocontrol strategies, (2) broadening the scope of grape breeding towards durable resistance (3) redesigning cropping systems to enhance prophylaxis and biodiversity (4) elaborating the structural alternatives and economic/regulatory incentives that will support the transition. VITAE also carries out foresight studies with stakeholders to generate scenarios for pesticide-free vine growing at the regional level. These scenarios will help organizations and policy makers to implement pesticide-free strategies with appropriate incentive programs.