Visible and near-infrared (Vis-NIR, 350–2500 nm) laboratory spectroscopy has been proven to provide soil properties estimations, such as clay or organic carbon (OC). However, the performances of such estimations may be dependent on pedological and spectral similarities between calibration and validation datasets. The objective of this study was to analyze how the soil order knowledge can be used to increase regression models performance for soil properties estimation. For this purpose, Random Forest regression models were calibrated and validated from both regional database (called regional models) and subsets stratified by soil order from the regional database (called soil-order models). The regional database contained 482 soil samples belonging to four soil orders (Alfisols, Vertisols, Inceptisols and Entisols) and associated with Vis-NIR laboratory spectra and six soil properties: OC, sand, silt, clay, cation exchange capacity (CEC) and pH. First, regional models provided i) high accuracy of some soil properties estimations when considering the regional strategy in the validation step (e.g., R2val of 0.74, 0.76 and 0.74 for clay, CEC and sand, respectively) but ii) modest accuracy of these same soil properties when considering subsets stratified by soil order from the regional database in validation step (e.g., R2val of 0.48, 0.58 and 0.38 over Vertisol for clay, CEC and sand, respectively). So the estimation accuracy appreciation is highly depending on the validation database as there is a risk of over-appreciated prediction accuracies at the soil-order scale when figures of merit are based on a regional validation dataset. Second, this work highlighted that the benefit of a soil-order model compared to a regional model for calibration depends on both soil property and soil order. So no recommendations for choosing between both models for calibration may be given. Finally, while Vis-NIR laboratory spectroscopy is becoming a popular way to estimate soil physicochemical properties worldwide, this work highlights that this technique may be used discreetly depending on the targeted scale and targeted soil type.