CD44v3 is a marker of invasive cancer stem cells driving metastasis in gastric carcinoma - Archive ouverte HAL Access content directly
Journal Articles (Data Paper) Gastric Cancer Year : 2022

CD44v3 is a marker of invasive cancer stem cells driving metastasis in gastric carcinoma

(1) , (1) , (2) , (3, 4, 1) , (1) , (1) , (1) , (1) , (5, 6, 7) , (1) , (8) , (1) , (1) , (9) , (4, 10, 1) , (4, 3) , (4, 10, 1) , (4, 3, 1) , (4, 3, 1) , (1)
1
2
3
4
5
6
7
8
9
10
Julie Pannequin

Abstract

Background: Cancer stem cells (CSCs) are at the origin of tumour initiation and progression in gastric adenocarcinoma (GC). However, markers of metastasis-initiating cells remain unidentified in GC. In this study, we characterized CD44 variants expressed in GC and evaluated the tumorigenic and metastatic properties of CD44v3+ cells and their clinical significance in GC patients. Methods: Using GC cell lines and patient-derived xenografts, we evaluated CD44+ and CD44v3+ GC cells molecular signature and their tumorigenic, chemoresistance, invasive and metastatic properties, and expression in patients-derived tissues. Results: CD44v3+ cells, which represented a subpopulation of CD44+ cells, were detected in advanced preneoplastic lesions and presented CSCs chemoresistance and tumorigenic properties in vitro and in vivo. Molecular and functional analyses revealed two subpopulations of gastric CSCs: CD44v3+ CSCs with an epithelial-mesenchymal transition (EMT)-like signature, and CD44+/v3- CSCs with an epithelial-like signature; both were tumorigenic but CD44v3+ cells showed higher invasive and metastatic properties in vivo. CD44v3+ cells detected in the primary tumours of GC patients were associated with a worse prognosis. Conclusion: CD44v3 is a marker of a subpopulation of CSCs with metastatic properties in GC. The identification of metastasis-initiating cells in GC represents a major advance for further development of anti-metastatic therapeutic strategies.
Fichier principal
Vignette du fichier
s10120-022-01357-y.pdf (2.92 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Licence : CC BY - Attribution

Dates and versions

hal-03908191 , version 1 (20-12-2022)

Licence

Attribution - CC BY 4.0

Identifiers

Cite

Julie Giraud, Lornella Seeneevassen, Benoit Rousseau, Damien Bouriez, Elodie Sifré, et al.. CD44v3 is a marker of invasive cancer stem cells driving metastasis in gastric carcinoma. Gastric Cancer, In press, ⟨10.1007/s10120-022-01357-y⟩. ⟨hal-03908191⟩
0 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More