The infiltration of secondary treated effluent (STE) into the soil downstream of wastewater treatment plants is becoming increasingly common in a climate change context. In STE infiltration, STE is discharged onto the soil over a large surface allowing for a gradual infiltration of the water. This paper investigates a novel time-lapse electrical resistivity tomography strategy to evaluate the impact of STE infiltration on the water pathways of two planted loamy-soil trenches located in a Fluvisol region in southwestern France. The system has been monitored for 3 years using discontinuous monitoring of electrical resistivity tomography during four saline tracer tests. Results show that: 1) the new methodology has successfully highlighted the evolution of water pathways in the soil over time; 2) such evolution is in agreement with reeds root distribution in the trenches which seems to be affected by water quality i.e. sludge losses and TSS, for this study case. Indeed, for the infiltration trench receiving STE with lower pollution levels (2.2 mg TSS. L-1, 26 mg COD. L-1), the infiltration capacity is maintained over the years (4-6 mm h-1) and reed roots developed deeper in the soil. A sludge deposit present at the bottom of the second infiltration trench receiving higher pollution levels (7.2 mg TSS. L-1, 45 mg COD. L-1, plus episodic sludge release) could lead roots to develop close to the surface affecting the infiltration capacity which did not evolve over time. This work highlights the importance of long-term flow pathway monitoring in understanding the hydraulic behavior of infiltration surfaces submitted to STE.