A yeast‐based tool for screening mammalian diacylglycerol acyltransferase inhibitors - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles MicrobiologyOpen Year : 2022

A yeast‐based tool for screening mammalian diacylglycerol acyltransferase inhibitors

Abstract

Dysregulation of lipid metabolism is associated with obesity and metabolic diseases but there is also increasing evidence of a relationship between lipid body excess and cancer. Lipid body synthesis requires diacylglycerol acyltransferases (DGATs) which catalyze the last step of triacylglycerol synthesis from diacylglycerol and acyl-coenzyme A. The DGATs and in particular DGAT2, are therefore considered potential therapeutic targets for the control of these pathologies. Here, the murine and the human DGAT2 were overexpressed in the oleaginous yeast Yarrowia lipolytica deleted for all DGAT activities, to evaluate the functionality of the enzymes in this heterologous host and DGAT activity inhibitors. This work provides evidence that mammalian DGATs expressed in Y. lipolytica are a useful tool for screening chemical libraries to identify potential inhibitors or activators of these enzymes of therapeutic interest.
Fichier principal
Vignette du fichier
2022_Gajdos_Microbio_Open.pdf (3.37 Mo) Télécharger le fichier
Origin Publisher files allowed on an open archive
Licence

Dates and versions

hal-04090516 , version 1 (05-05-2023)

Licence

Identifiers

Cite

Peter Gajdoš, Rodrigo Ledesma‐amaro, Jean‐marc Nicaud, Tristan Rossignol. A yeast‐based tool for screening mammalian diacylglycerol acyltransferase inhibitors. MicrobiologyOpen, 2022, 11 (6), pp.e1334. ⟨10.1002/mbo3.1334⟩. ⟨hal-04090516⟩
42 View
5 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More