Molecular characterisation of human adenoviruses associated with respiratory infections in Uganda - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles BMC Infectious Diseases Year : 2023

Molecular characterisation of human adenoviruses associated with respiratory infections in Uganda

Abstract

Human adenoviruses (HAdV) are a diverse group of viruses causing a broad range of infections of the respiratory, urogenital and gastrointestinal tracts and keratoconjunctivitis. There are seven species of human adenoviruses with 113 genotypes which may contain multiple genetic variants. This study characterised respiratory human adenoviruses and associated factors in samples collected from selected hospitals in Uganda. A total of 2,298 nasopharyngeal samples were collected between the period of 2008 to 2016 from patients seeking health care at tertiary hospitals for influenza-like illness. They were screened by polymerase chain reaction (PCR) to determine the prevalence of HAdV. HAdV was cultured in A549 cell lines and the hexon gene was sequenced for genotyping. Of the 2,298 samples tested, 225 (9.8%) were adenovirus-positive by PCR. Age was found to be significantly associated with HAdV infections (p = 0.028) with 98% (220/225) of the positives in children aged 5 years and below and none in adults above 25 years of age. The sequenced isolates belonged to species HAdV-B and HAdV-C with most isolates identified as genotype B3. The results showed a high prevalence and genetic diversity in respiratory HAdV circulating in Ugandan population. Deeper genomic characterization based on whole genome sequencing may be necessary to further elucidate possible transmission and impact of current adenovirus-vectored vaccines in Africa.
Fichier principal
Vignette du fichier
s12879-023-08403-9.pdf (2.03 Mo) Télécharger le fichier
Origin Publisher files allowed on an open archive
Licence

Dates and versions

hal-04148208 , version 1 (02-07-2023)

Licence

Identifiers

Cite

Qouilazoni A. Ukuli, Bernard Erima, Andrew Mubiru, Gladys Atim, Titus Tugume, et al.. Molecular characterisation of human adenoviruses associated with respiratory infections in Uganda. BMC Infectious Diseases, 2023, 23, pp.435. ⟨10.1186/s12879-023-08403-9⟩. ⟨hal-04148208⟩
6 View
3 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More