Fear of humans can negatively impact offspring's' behaviour and cognition whereas early positive handling has positive impacts. The aim of this study was to evaluate the crossed effects of trans-generational transfer of fear of humans and familiarization with an experimenter on piglets' behaviour. Sows were classified into two categories (Fearful, n=11 and Docile, n=13) based on whether they avoided human contact or not during a human approach test at 71 days of gestation. At 72 days of gestation, saliva collection was done by an operator inserting a salivette in sows' mouth so that they could chew during 10 seconds. Salivary cortisol concentra- tions were measured by doubled luminescence luminoassay. Fear of humans wasn't related to salivary cortisol levels of the sows (F =2.72, P=0.10), sampled. Fearful sows' piglets stood immobilized for longer than docile sow's piglets (Chisq= 6.24, P=0.01), and females stood immobilized for longer than males (8.70, P=0.003) during a tonic immobility test (TIT) at 7 days but not at 15 days. The behaviour of two male and two female piglets per sow (n=96) was studied after weaning (28 days). We found no effect of sow's reaction to the experimenter on piglets' emotionality expressed during an open-field test (OFT) at 32-33 days. However, during a Human volun- tary approach test (HVAT) piglets born from fearful sows were more fearful than piglets born from docile sows (Chisq=8.23, P=0.004,). Half of the piglets (n=48, 1 male and 1 female per sow) were then familiarized with an experimenter while the others received minimum contact. We found no main or crossed effect of sow's reac- tion to the experimenter and piglet's treatment on piglets' emotionality during an OFT, nor on their exploratory behaviour during HVAT at 46-47 days. Piglets' born from fearful sows were less attracted to the experimenter during HVAT2 (Chisq=8.33, P=0.004) and familiarized piglets were more attracted (Chisq=15.05, P<0.001). Piglets born from docile sows found the reward faster in a spatial maze test than those born from fearful sows. Both sows perception of humans (fearful or docile) and piglet familiarization with an experimenter seemed to specif- ically affect humans related behaviours without affecting their general emotionality, except in the spatial maze test.