Soybean meals (SMB) fed to dairy cows are obtained through mechanical and/or chemical extraction of oil from the seed. Chemical extraction consists of solvent extraction, extraction-grade hexane being the most used solvent in the industry. Concerns regarding the health effects of n-hexane residues in food and feed are driving the industry to find alternatives to extraction-grade hexane. 2-methyloxolane (2-meOx) could be one of these. However, process differences between hexane and 2-meOx defatted SBM may lead to differing nutritive values and may affect the performance of dairy cows. A 4×4 Latin-square experiment with 16 primiparous lactating Holstein cows was conducted to study the effects of 2-meOx on milk yield and composition, nitrogen use efficiency for milk production, and solvent residues in milk. Cows were fed a diet containing 14% SBM and 165 g/kg DM crude protein. The 4 treatments differed according to the inclusion level of hexane and 2-meOx defatted SBM (i.e. 100% hexane, 66% hexane – 33% 2-meOx, 33% hexane and 66% 2-meOx, and 100% 2-meOx). Results show that dry matter intake, milk yield, milk protein concentration and excretion, milk protein fractions, and milk fat excretion did not differ among treatments. Milk fat concentration tended to increase with 33% hexane – 66% 2-meOx compared to 100% hexane SBM (P=0.07). Nitrogen intake, milk nitrogen excretion and nitrogen use efficiency for milk production did not differ according to the treatment. N-hexane residues tended to be more often detected in the milk from 100% hexane-fed cows compared to 100% 2-meOX-fed ones (P=0.08). 2-meOx residues were only detected in milk from 2-meOx-fed cows, but the difference with 100% hexane-fed cows was not significant. When detected in the milk, n-hexane and 2-meOx concentrations were below the quantification threshold of 10 ng/g. These preliminary results indicate that 2-meOx-defatted SBM can replace hexane-defatted SBM without affecting milk production performance and nitrogen use efficiency. Further analyses are required to confirm results regarding the detection of solvent residues in milk.