Testing minimally invasive blood collection techniques in pigs used for research - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Conference Papers Year : 2023

Testing minimally invasive blood collection techniques in pigs used for research

Test de techniques peu invasives pour collecter du sang chez des porcs en expérimentation

F A Eugenio
  • Function : Author
M Oster
  • Function : Author
C Ollagnier
  • Function : Author


The collection of blood to monitor circulating concentrations of metabolites is an important tool for research to better describe the nutritional and health status of animals. In pigs, venepuncture requires the animal to be restrained, which can be stressful and painful, and large veins are also not very visible which complicates the procedure. An alternative is catheterization, but this involves a surgical procedure which induces an inflammatory response that can mask certain metabolic responses, and needs the housing of pigs in isolated pens. This study aimed to test alternative blood collection procedures and associated scaled down methods to assess circulating metabolites in growing pigs. In Exp 1, four blood collection methods were tested in pigs (n=6 females, 61 kg body weight): (1) catheterization; (2) venepuncture with the aid of a handheld infra-red imaging tool; (3) ear pricking with a lancet to collect drops of blood in filter papers; and (4) blood sucking Dipetalogaster maxima parasites. Blood collection was done at four time points using each method in a randomised order after feeding the pigs a liquid glucose meal. Methods were compared for the relative ease of collection, animal stress indicators, and obtained concentrations of cortisol and glucose. In Exp 2, glucose sensors with an intradermal needle were secured on the neck of pigs (n=8 females, 50 kg body weight); pigs were also equipped with a jugular catheter to serve as a control. Circulating glucose concentrations analysed by the sensor were obtained during five days with two diets (high starch then a high fat). Both basal concentrations and postprandial glucose curves during four hours after two meal test procedures were also compared with those obtained by blood sampling through the catheter. Ongoing laboratory and data analysis will reveal the best suitable approach in minimising the negative impact of blood collection in pigs used in experimental research.
Fichier principal
Vignette du fichier
abstract-EAAP-F Aman Eugenio.pdf (756.27 Ko) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-04205484 , version 1 (12-09-2023)


  • HAL Id : hal-04205484 , version 1


F A Eugenio, F Gondret, M Oster, C Ollagnier. Testing minimally invasive blood collection techniques in pigs used for research. 74th annual meeting of the european federation of animal science, EAAP, Aug 2023, LYON, France. pp.473. ⟨hal-04205484⟩
0 View
0 Download


Gmail Facebook Twitter LinkedIn More