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Article Dans Une Revue Methods in Molecular Biology Année : 2021

Confocal Laser Microscopy Analysis of Listeria monocytogenes Biofilms and Spatially Organized Communities

Résumé

The behavior of Listeria monocytogenes communities in the food chain is closely associated with their spatial organization. Whether as biofilms on industrial surfaces or as microcolonies in food matrices, the resulting physiological diversification combined with the presence of extracellular polymeric substances (EPS) triggers emergent community functions involved in the pathogen survival and persistence (e.g., tolerance to dehydration, biocides, or preservatives). In this contribution, we present a noninvasive confocal laser microscopy (CLM) protocol allowing exploration of the spatial organization of L. monocytogenes communities on various inert or nutritive materials relevant for the food industry.

Dates et versions

hal-04272118 , version 1 (06-11-2023)

Identifiants

Citer

Célia Sahli, Sergio Moya, John Lomas, Christine Gravier-Pelletier, Romain Briandet, et al.. Confocal Laser Microscopy Analysis of Listeria monocytogenes Biofilms and Spatially Organized Communities. Methods in Molecular Biology, 2021, Methods in Molecular Biology, 2220 (5), pp. 123-136. ⟨10.7150/thno.67296⟩. ⟨hal-04272118⟩
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