Differential Gene Expression Associated with Soybean Oil Level in the Diet of Pigs
Simara Larissa Fanalli
(1)
,
Bruna Pereira Martins da Silva
(1)
,
Julia Dezen Gomes
(1)
,
Vivian Vezzoni de Almeida
(2)
,
Felipe André Oliveira Freitas
(1)
,
Gabriel Costa Monteiro Moreira
(3)
,
Bárbara Silva-Vignato
(4)
,
Juliana Afonso
(5)
,
James Reecy
(6)
,
James Koltes
(6)
,
Dawn Koltes
(6)
,
Luciana Correia de Almeida Regitano
(5)
,
Dorian John Garrick
(7)
,
Júlio Cesar de Carvalho Balieiro
(4)
,
Ariana Nascimento Meira
(1)
,
Luciana Freitas
(8)
,
Luiz Lehmann Coutinho
(1)
,
Heidge Fukumasu
(4)
,
Gerson Barreto Mourão
(1)
,
Severino Matias de Alencar
(1)
,
Albino Luchiari Filho
(1)
,
Aline Silva Mello Cesar
(1)
1
ESALQ -
Escola Superior de Agricultura "Luiz de Queiroz"
2 College of Veterinary Medicine and Animal Science, Federal University of Goiás, Goiânia, 74001-970, GO, Brazil
3 GIGA-Research - Groupe Interdisciplinaire de Génoprotéomique Appliquée
4 FZEA Faculty of Animal Science and Food Engineering
5 Embrapa - Brazilian Agricultural Research Corporation
6 ISU - Iowa State University
7 AL Rae Centre for Genetics and Breeding, Massey University, Hamilton
8 DB Genética de Suínos, Patos de Minas 38706-000, MG
2 College of Veterinary Medicine and Animal Science, Federal University of Goiás, Goiânia, 74001-970, GO, Brazil
3 GIGA-Research - Groupe Interdisciplinaire de Génoprotéomique Appliquée
4 FZEA Faculty of Animal Science and Food Engineering
5 Embrapa - Brazilian Agricultural Research Corporation
6 ISU - Iowa State University
7 AL Rae Centre for Genetics and Breeding, Massey University, Hamilton
8 DB Genética de Suínos, Patos de Minas 38706-000, MG
Simara Larissa Fanalli
- Fonction : Auteur
- PersonId : 1379184
- ORCID : 0000-0002-8140-0721
Felipe André Oliveira Freitas
- Fonction : Auteur
- PersonId : 1379190
- ORCID : 0000-0002-8168-7144
Gabriel Costa Monteiro Moreira
- Fonction : Auteur
- PersonId : 1361608
- IdHAL : gabriel-costa-monteiro-moreira
- ORCID : 0000-0003-3139-1027
Bárbara Silva-Vignato
- Fonction : Auteur
- PersonId : 1379188
- ORCID : 0000-0001-9796-8007
Juliana Afonso
- Fonction : Auteur
- PersonId : 1379189
- ORCID : 0000-0002-4435-5386
Dorian John Garrick
- Fonction : Auteur
- PersonId : 1011528
- ORCID : 0000-0001-8640-5372
Heidge Fukumasu
- Fonction : Auteur
- PersonId : 1379277
- ORCID : 0000-0001-9865-8983
Gerson Barreto Mourão
- Fonction : Auteur
- PersonId : 1379195
- ORCID : 0000-0002-0990-4108
Severino Matias de Alencar
- Fonction : Auteur
- PersonId : 1379199
- ORCID : 0000-0002-6637-7973
Aline Silva Mello Cesar
- Fonction : Auteur
- PersonId : 1379201
- ORCID : 0000-0001-9921-214X
Résumé
The aim of this study was to identify the differentially expressed genes (DEG) from the skeletal muscle and liver samples of animal models for metabolic diseases in humans. To perform the study, the fatty acid (FA) profile and RNA sequencing (RNA-Seq) data of 35 samples of liver tissue (SOY1.5, n = 17 and SOY3.0, n = 18) and 36 samples of skeletal muscle (SOY1.5, n = 18 and SOY3.0, n = 18) of Large White pigs were analyzed. The FA profile of the tissues was modified by the diet, mainly those related to monounsaturated (MUFA) and polyunsaturated (PUFA) FA. The skeletal muscle transcriptome analysis revealed 45 DEG (FDR 10%), and the functional enrichment analysis identified network maps related to inflammation, immune processes, and pathways associated with oxidative stress, type 2 diabetes, and metabolic dysfunction. For the liver tissue, the transcriptome profile analysis revealed 281 DEG, which participate in network maps related to neurodegenerative diseases. With this nutrigenomics study, we verified that different levels of soybean oil in the pig diet, an animal model for metabolic diseases in humans, affected the transcriptome profile of skeletal muscle and liver tissue. These findings may help to better understand the biological mechanisms that can be modulated by the diet.