Methods for obtaining nucleotide sequences coding for polypeptides specifically active for larvae of S. littoralis - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Patents Year : 2005

Methods for obtaining nucleotide sequences coding for polypeptides specifically active for larvae of S. littoralis

Abstract

This invention provides a method for obtaining a nucleotide sequence that codes for the active part of a polypeptide specifically active for larvae of S. littoralis. The method comprises hybridizing at least one nucleotide probe to DNA from a strain of B. thuringiensis active against S. littoralis, wherein at least one nucleotide probe is derived from the 5' part of a restriction fragment of a gene for delta-endotoxin of B. thuringiensis strain aizawa 7-29, isolating the DNA from the strain of B. thuringiensis active against S. littoralis that hybridized to the probe, cloning the isolated DNA into a vector, and purifying the vector to thereby obtain the nucleotide sequence that codes for the active part of a polypeptide specifically active for larvae of S. littoralis, wherein the specifically active polypeptide has (1) a specificity index of less than 1.0 and (2) a higher specific toxic activity towards S. littoralis than the native crystal proteins of the strains aizawai 7-29 or berliner 1715.
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Dates and versions

hal-04617169 , version 1 (19-06-2024)

Identifiers

  • HAL Id : hal-04617169 , version 1

Cite

Vincent Sanchis, Didier Lereclus, Ghislaine Menou, Marguerite-Marie Lecadet, Daniel Martouret, et al.. Methods for obtaining nucleotide sequences coding for polypeptides specifically active for larvae of S. littoralis. France, Patent n° : 20050091714. 2005. ⟨hal-04617169⟩

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