A clade of receptor-like cytoplasmic kinases and 14-3-3 proteins coordinate inositol hexaphosphate accumulation - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Journal Articles Nature Communications Year : 2024

A clade of receptor-like cytoplasmic kinases and 14-3-3 proteins coordinate inositol hexaphosphate accumulation

Li Lin Xu
  • Function : Author
Miao Jing Zhang
  • Function : Author
Gui Xin Li
  • Function : Author
Ji Ming Xu
  • Function : Author
Xiao Dan Wu
  • Function : Author
Chuan Zao Mao
Wo Na Ding
  • Function : Author
  • PersonId : 1405620
Zhong Jie Ding

Abstract

Abstract Inositol hexaphosphate (InsP 6 ) is the major storage form of phosphorus in seeds. Reducing seed InsP 6 content is a breeding objective in agriculture, as InsP 6 negatively impacts animal nutrition and the environment. Nevertheless, how InsP 6 accumulation is regulated remains largely unknown. Here, we identify a clade of receptor-like cytoplasmic kinases (RLCKs), named Inositol Polyphosphate-related Cytoplasmic Kinases 1-6 (IPCK1-IPCK6), deeply involved in InsP 6 accumulation. The InsP 6 concentration is dramatically reduced in seeds of ipck quadruple ( T-4m / C-4m ) and quintuple ( C-5m ) mutants, accompanied with the obviously increase of phosphate (Pi) concentration. The plasma membrane-localized IPCKs recruit IPK1 involved in InsP 6 synthesis, and facilitate its binding and activity via phosphorylation of GRF 14-3-3 proteins. IPCKs also recruit IPK2s and PI-PLCs required for InsP 4 /InsP 5 and InsP 3 biosynthesis respectively, to form a potential IPCK-GRF-PLC-IPK2-IPK1 complex. Our findings therefore uncover a regulatory mechanism of InsP 6 accumulation governed by IPCKs, shedding light on the mechanisms of InsP biosynthesis in eukaryotes.
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hal-04665341 , version 1 (31-07-2024)

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Li Lin Xu, Meng Qi Cui, Chen Xu, Miao Jing Zhang, Gui Xin Li, et al.. A clade of receptor-like cytoplasmic kinases and 14-3-3 proteins coordinate inositol hexaphosphate accumulation. Nature Communications, 2024, 15 (1), pp.5107. ⟨10.1038/s41467-024-49102-6⟩. ⟨hal-04665341⟩
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