Poster De Conférence Année : 2023

Molecular dialogue of plant-bacterial interactions during seed transmission

Résumé

Seed is the vector of dispersion of many phytopathogenic bacteria. The molecular dialogue that is established between the plant and these microorganisms during their transmission remains largely unknown, mainly due to the technological lock of isolating bacterial RNA from infected seeds. In this project, RNA sequencing technologies were used on both plant and bacterial cells in complement to capture technologies for enrichment of specific bacterial RNA. Changes in bacterial and plant transcriptomes, but also plant methylomes were investigated during seed transmission of the plant pathogenic bacteria Xanthomonas citri pv. fuscans (Xcf) on common bean (Phaseolus vulgaris). During the seed filling stage, we observed major transcriptomic changes in both partners. In particular a concomitant differential expression of plant defense genes (e.g. serine peptidases, LRR-kinases, MAPKs, regulators such as bZIP, TIFY, AP2/ERF) and bacterial type II/type III secretion systems effectors occurred during this stage. This intense pathogenic/defense molecular dialogue at early seed developmental stage was shut down in latter stages during seed maturation, and at maturity. We also noted that at maturity, seed transmission of Xcf altered DNA methylation at more than 800 plant genomic regions. These changes included methylation changes within promoter or coding regions of genes involved in plant defense (e.g. WRKY, NB-LR or PUB13) and seed development (e.g. AGL62, FIE2 or LEA proteins). Deep-sequencing of small RNAs at the filling and mature seed stages highlighted potential miRNA (30%) and siRNA (44%) populations originated from P. vulgaris genome. The perspectives are now to integrate all these data to identify which plant miRNAs can mediate transcript repression in plant and bacterial transcriptomes and which plant siRNA can mediate change in plant methylome.
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Dates et versions

hal-04665363 , version 1 (31-07-2024)

Identifiants

  • HAL Id : hal-04665363 , version 1

Citer

Armelle Darrasse, Łukasz Paweł Tarkowski, Martial Briand, David Lalanne, Nicolas W. G. Chen, et al.. Molecular dialogue of plant-bacterial interactions during seed transmission. Rencontres Plantes Bactéries Aussois, 2023, Aussois, France. ⟨hal-04665363⟩
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