Lavender (Lavandula angustifolia L.) is a patrimonial and economically important species in Southern part of Europe. However, increasing pathogenic pressure related to climate change challenges cultivated varieties yields and quality of essential oils. The cultivar ‘Maillette’, that is most-valued for the quality of its essential oil, is widely used in genetic improvement programs in France. We previously generated a long-reads based genome assembly from an individual of the lavender ‘Maillette’ using PacBio HiFi data (579 contigs assembled, representing 891.5 Mb, N50=20.35Mb, QV score of 41.8 and 97.1% of complete BUSCO genes). Herein we built a genetic map for ‘Maillette’, using a genotyping tool we developed. This linkage map (822 polymorphic SNP ~33 SNP per linkage group) encompassed the expected 25 linkage groups. This linkage map was then used to scaffold our assembly. Fifty-four contigs were grouped in 25 pseudomolecules (N50=34.78 Mb) containing 94.9% (846.2/891.5 Mb) of the assembly, plus 551 unplaced contigs (min=17.8 kb ; max= 3.8 Mb). Twenty-two of twenty-five chromosomes showed telomere repeats at either both ends (11 chromosomes) or one end (11 chromosomes). Benefiting from available genome sequences showing different chromosome numbers, we highlighted the good synteny between Maillette (25 chromosomes) and Munstead (25 chromosomes) and identified 2 large chromosomal fissions (in Maillette chr03 and chr21) differentiating Maillette from JingXu2 (27 chromosomes). This chromosome-level assembly for an economically important variety of this emblematic species will allow a better estimates of the cultivar diversity and benefit to the improvement programs through reliable GWAS.