Organoids are in vitro models derived from stem cells that recapitulate many aspects of the complex structure and function of the corresponding in vivo tissue. We previously characterized organoid cultures from different segments of the porcine gut vs the original tissue by RNA seq. A vast majority of expressed genes was shared between organoids and their tissue counterparts at low TPM thresholds At higher TPM, innate immune and epithelial gene lists maintained a higher proportion of shared genes vs the full gene catalogue, confirming that organoids are a good in vitro 3 Rs” proxy to evaluate genotype to phenotype relationships involving innate immunity responses of the gut epithelia. We aim to optimize cutting edge genome editing tools and protocols on pig intestinal organoids for our host virus interactionstudies As a proof of concept, we will generate knock out organoids for the ANPEP gene, a well known receptor of a porcine virus (TGEV). We report here preliminary results on the transfection efficiency of porcine gut organoids by electroporation of a fluorescent reporter plasmid, adapting the method described for human organoids (Fuji et al, 2015).