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                <term xml:lang="fr">culture de cellules</term>
                <term xml:lang="fr">comptage de noyaux</term>
                <term xml:lang="fr">myotube</term>
                <term xml:lang="fr">myoblaste</term>
                <term xml:lang="fr">noyau</term>
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              <p>En culture de cellules musculaires, les cellules ont la particularité, lors de leur différenciation, de fusionner entre elles pour former des myotubes, précurseurs des fibres musculaires qui ont la faculté de se contracter. Deux des principales données étudiées sont les indices de prolifération et de différenciation. La mesure de ces indices se fait généralement par comptage manuel sous microscope des noyaux totaux et des noyaux présents dans les myotubes. Ces comptages étant longs et fastidieux, nous les avons automatisé à l'aide du logiciel Visilog 6.7 ( Noesis, France). Cette nouvelle méthode de comptage donne des résultats plus représentatifs de l'état de différenciation des cultures et de plus, elle permet de mesurer la taille des myotubes.</p>
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