Bread wheat (Triticum aestivum) is mainly used after transformations. All these transformations require a given end-use quality, which depends on seed storage protein (SSP) concentration and composition. Expression of SSP genes is mainly regulated by transcription factors (TFs), which specifically bind cis-motifs in the promoter region. This first level of the transcriptional regulation of SSP genes implies TFs able to bind cis-motifs included in their promoter. In barley, eight TFs are involved in this regulation. In wheat, all these TFs are found (SPA, SHP, PBF, SAD, GAMYB, MYBS3, MCB1 and FUSCA3). Five of them have been demonstrated to regulate SSP synthesis. These TFs could be also regulated by transcriptional proteins (second level of regulation). Currently, knowledge on the TFs able to modulate the expression of genes coding for SPA, SHP, PBF, SAD, GAMYB, MCB1, MYBS3 and FUSCA3 is poor. Therefore, we aimed at identifying the TFs involved in the second level of regulation (called candidate TFs), studying their polymorphism using exome data provided by Whealbii project (https://www.whealbi.eu/fr/). Polymorphisms were then used to study their effects on the SSP composition content and composition by association mapping.