Effect of Simulated Micro and Hyper-Gravity on Gene Fitness in Bacterial Biofilms
Résumé
Biofilms are surface-associated microbial communities embedded in a self-produced extracellular polymeric substance (EPS) (1). These structures provide cells with emergent properties, such as enhanced tolerance to antimicrobials and biocide treatments. In space environment, these microbial communities threaten lifesupport systems, including the water recovery system aboard the International Space Station (ISS), and pose risks to astronaut health. We have developed a CRISPRi-mediated gene silencing approach to address these challenges and identify key genes essential for biofilm formation under altered gravity. A CRISPRi library targeting upregulated genetic elements under biofilm conditions was created using existing transcriptomic data on the biofilm-forming strain Bacillus subtilis NDmed (2,3). The aim was to perform CRISPRi pool screening to gain insight into the mechanisms underlying biofilm formation under altered gravity.
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