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Theses

Reproduction de l'esturgeon sibérien (Acipenser baeri Brandt) en élevage : gestion des génitrices, compétence à la maturation in vitro de follicules ovariens et caractéristiques plasmatiques durant l'induction de la ponte

Abstract : This work is related to the final stages of the reproduction of cultured Siberian strugeon. Hormonal stimulation is needed to obtain the maturation, the ovulation and the spawning. Rearing conditions as well as ovarian cycles within a cohort throughout a 5 years studied period are described. Selection of spawners suitable to be hormonally treated is carried out in 2 stages. The first occured in Autumn and consist in isolating the gravid spawners of which the minimum follicle diameter is 2,8 mm. The second is practised just before reproduction depending on morpho-physiological criteria of oocyte follicles of which the evolution throughout the potential reproductive period is described. The work with the physiological criteria needed special research related to the in vitro incubation media used for oocyte follicles. The best media proved to be that based on plasmatic characteristics of Siberian sturgeon. The most efficient criteria to select the spawners is the determination of 50% time course for in vitro maturation of oocyte follicles induced by progestagen. There are no significant difference in reproductive performance whatever the pituitary extract used in vivo, carp or sturgeon. The cannulation of spawners throughout spawning allowed to establish plasmatic profiles for ions, stress indicators and following sexual steroids ; testosterone, 11-ketotestosterone, 17alpha,20beta-dihydroxyprogesterone et estradiol.
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https://hal.inrae.fr/tel-02576138
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Submitted on : Thursday, May 14, 2020 - 5:37:13 PM
Last modification on : Tuesday, September 1, 2020 - 2:08:03 PM

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  • HAL Id : tel-02576138, version 1
  • IRSTEA : PUB00002943

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Patrick Williot. Reproduction de l'esturgeon sibérien (Acipenser baeri Brandt) en élevage : gestion des génitrices, compétence à la maturation in vitro de follicules ovariens et caractéristiques plasmatiques durant l'induction de la ponte. Sciences de l'environnement. Doctorat Sciences biologiques et médicales, Université Bordeaux I, 1997. Français. ⟨tel-02576138⟩

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