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Finding loss-of-susceptibility resistance genes toward Tospoviruses in Solanaceae

Abstract : The Tomato spotted wilt virus (TSWV) is the second most destructive plant virus, particularly in Solanaceae. Because the resistance genes to TSWV available in tomato (Solanum lycopersicum) and pepper (Capsicum annuum) are overcome by resistance breaking isolates, there is an urgent need to develop new durable genetic resistance. Resistance by loss-of-susceptibility is one of the great opportunities to provide such resistances. The aim of this present work is first to characterize a TSWV isolate and then identify tomato host proteins that interact with the viral proteins, aiming at characterizing new resistances. - Firstly, Tomato spotted wilt virus RNA-dependent RNA polymerase adaptive evolution and most constrained domains was analyzed. The TSWV-LYE51 genome was sequenced. We decided to focus on the RNA-dependent RNA polymerase which is the key protein for cycle life by being responsible for both the transcription and replication of the viral genome. A 3D model of this RdRp protein was built by homology modeling and phylogenetic and selection analyses were used to characterize hot-spot of adaptive mutation as well as the most constrained domains. Those results allow to pinpoint RdRP regions of interest toward which efficient resistances can be targeted. - Based on those data, candidates genes implicated in susceptibility to TSWV were sought after. Viruses have to hijack host cellular protein –i.e. named susceptibility factors- in order to accomplish their cycle and infect their host. Those factors can be turned into genetic resistance through their absence or modification. To characterize such susceptibility factors in tomato, yeast-two hybrid screenings were carried out using the identified RdRP constrained domain as a bait. We identify several candidates’ genes currently being validated. This work, by obtaining new information about TSWV infectious mechanism and its adaptation to plan, may bring new perspectives in order to develop loss-of-susceptibility resistances to TSWV in crops by using natural or induced variability.
Keywords : Tospovirus TSWV
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https://hal.inrae.fr/tel-02790682
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Submitted on : Friday, June 5, 2020 - 6:44:43 AM
Last modification on : Thursday, December 3, 2020 - 4:46:03 PM

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  • HAL Id : tel-02790682, version 1
  • PRODINRA : 496136

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Zoé Terret. Finding loss-of-susceptibility resistance genes toward Tospoviruses in Solanaceae. Agricultural sciences. Aix Marseille Université, 2019. English. ⟨tel-02790682⟩

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