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, peut présenter une alternative prometteuse en association avec d'autres méthodes dans le cadre d'une protection intégrée des cultures. L'objectif de cette thèse a été d'étudier l'effet des rayonnements UV-C sur la sensibilité des plantes aux agents pathogènes. Les rayonnements UV-C ont été appliqués durant la culture, seuls ou en combinaison à des agents de biocontrôle, ou en post-récolte. Les expérimentations ont été menées sur deux espèces végétales : le fraisier et la tomate. Sur fraisier, des traitements UV-C à une dose de 1,70 kJ/m² entraînent une diminution systématique d'environ 20 % de la sensibilité des feuilles à Botrytis cinerea et diminuent jusqu'à 50 % le nombre de feuilles attaquées par Podosphaera aphanis, l'agent causal de l'oïdium. Chez la tomate, la protection des feuilles contre B. cinerea atteint 43 % avec une dose d'UV-C cumulée de 4,0 kJ/m². Ces mêmes traitements UV-C appliqués sur fruits réduisent la sensibilité des fraises vis-à-vis de Rhizopus de 75 % mais aucun effet n'est observé vis-à-vis de B. cinerea. Au contraire, sur fruits de tomate, la sensibilité est diminuée d'environ 63 % contre cet agent pathogène. Parallèlement, des tests biochimiques et gustatifs suggèrent que la qualité des fruits n'est pas significativement améliorée ni altérée par les expositions UV-C. Malgré un effet germicide marqué des rayonnements UV-C sur feuilles de fraisiers, aucun effet bénéfique de l'application de ces rayonnements sur le niveau d'efficacité d'agents de lutte biologique n'a été mis en évidence. Nos observations sont encourageantes pour envisager une utilisation de cette méthode dans un schéma de protection intégrée des cultures. Cependant, Certains champignons sont très dommageables pour les plantes mais aussi pour la conservation après récolte des produits végétaux. Les restrictions réglementaires concernant l'utilisation des fongicides nécessitent de trouver des solutions alternatives. L'amélioration de la résistance des plantes en utilisant des méthodes physiques, comme les rayonnements UV

. Mots, B. Uv-c, and . Cinerea, Improving plant resistance using physical methods, such as aplication of UV radiation, can be a promising alternative in association with other methods as part of an integrated pest management strategy. The objective of the present thesis was to study the effect of UV-C aplication on the sensitivity of plants to fungal pathogens. UV-C radiation was applied during plant cultivation, alone or in combination with biocontrol agents, or in post-harvest. Experiments were conducted on two plant species: strawberry and tomato. On strawberry plants, UV-C treatments at 1.70 kJ/m² result in a systematic 20 % decrease in leaves sensitivity to Botrytis cinerea and in a reduction by up to 50 % of the number of leaves infected by Podosphaera aphanis, the causal agent of powdery mildew. In tomato, leaves protection against B. cinerea reaches 43 % with a cumulative dose of 4.0 kJ/m². UV-C treatments applied on fruits reduce the strawberry sensitivity to Rhizopus by 75 % but no effect is observed against B. cinerea. On the contrary, sensitivity against B. cinerea is reduced by 63 % on tomato fruit. Biochemical and taste tests suggest that fruit quality is not significantly improved or altered by UV-C treatment. Despite a marked germicidal effect of UV-C radiation on strawberry leaves, it has no significant effect on the protective efficacy of biocontrol agents. Our observations are encouraging to consider the use of this tool in an integrated crop protection scheme. However, a clear understanding of how UV-C radiations are perceived by plants and their translation, Podosphaera aphanis, Rhizopus, biocontrôle, qualité des fruits Abstract Some fungi are very damaging during plant cultivation and also in post-harvest storage of products. Regulatory restrictions on fungicide use require alternative solutions