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, At-eIF4E3 81 KSNQVIWGSSLRSLYTFGTIEEFWSLYNNIHPPTKWVSGADLYCFKDKIEPKWEDPICANGGKWSMMFPK---ATLECNW At-eIF4E1 76 KSKQTSWGSSLRPVFTFSTVEEFWSLYNNMKHPSKLAHGADFYCFKHIIEPKWEDPICANGGKWTMTFPK---EKSDKSW At-eIF(iso4E) 42 KG--AAWGASLRKAYTFDTVEDFWGLHETIFQTSKLTANAEIHLFKAGVEPKWEDPECANGGKWTWVVTANRKEALDKGW At-nCBP 59 GVRNQSYEDNIKKMVEFSTVEGFWACYCHLARSSLLPSPTDLHFFKDGIRPLWEDGANCNGGKWIIRFSK---VVSARFW consensus 81, At-eIF4E2, vol.81

*. *. ,

*. *. ,

*. .. , * At-eIF4E2 158 LNTLLALVGEQFDQGDEICGAVLNFR--TRGDRISLWTKKAANEEAQLSIGKQWKELLGYNDTIGFIVHEDAKTLDRDAK At-eIF4E3 158 LNTLLALVGEQFDQGDEICGAVLNFR--ARGDRISLWTKNAANEEAQLSIGKQWKELLGYNETIGFIVHEDAKTLDRDAK At-eIF4E1, p.153

. At-eif, -R At-nCBP 136 EDLLLALVGDQLDDADNICGAVLSVR--FNEDIISVWNRNASDHQAVMGLRDSIKRHLKLPHAYVMEYKPHDASLRDNSS consensus 161, p.120

, At-eIF4E2 236 RRYTV---At-eIF4E3 236 RRYTV---At-eIF4E1 231 NAYTA---At-eIF(iso4E) 194 SRFTV---At-nCBP 214 YRNTWLRG consensus 241

, Sensibilisation des plaques : dépôt des anticorps dans les puits d'une plaque

, Fixation de l'antigène (protéines virales extraites des échantillons de feuilles broyés) aux anticorps

, Révélation : dépôt du substrat de la phosphatase alcaline (PNPP)

, S'il y a réaction antigène-anticorps, la phosphatase alcaline dégrade le substrat, ce qui se traduit par une réaction colorimétrique