Premise of the study: Microsatellite markers for Centella asiatica , an important medicinal herb, were developed and character- ized to promote genetic and molecular studies. * Methods and Results: A GA/GT-enriched genomic library was constructed from an accession from Madagascar. Roughly 75% of the 768 clones of the enriched library contained microsatellites. Eighty sequences containing microsatellites were obtained from 96 positive clones. Specifi c primers were designed for 20 loci, and 17 of them displayed polymorphism when screened across 17 C. asiatica accessions, with an average of 4.3 alleles per locus . The observed and expected heterozygosity values averaged 0.114 and 0.379, respectively. * Conclusions: This is the fi rst report constructing an enriched genomic library and identifying microsatellite markers from C. asiatica . These 17 polymorphic microsatellite markers are a useful resource for this plant, applicable for diversity studies, pedigree analyses, and genetic mapping.