Identification of molecular markers for DNA barcoding in the Aphidiinae (Hym. Braconidae) - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Molecular Ecology Resources Année : 2012

Identification of molecular markers for DNA barcoding in the Aphidiinae (Hym. Braconidae)

S.A.P. Derocles
  • Fonction : Auteur
B. Chaubet
  • Fonction : Auteur
C. Cruaud
A. Cruaud
  • Fonction : Auteur
J.Y. Rasplus

Résumé

Reliable identification of Aphidiinae species (Braconidae) is a prerequisite for conducting studies on aphidparasitoid interactions at the community level. However, morphological identification of Aphidiinae species remains problematic even for specialists and is almost impossible with larval stages. Here, we compared the efficiency of two molecular markers [mitochondrial cytochrome c oxydase I (COI) and nuclear long wavelength rhodopsin (LWRh)] that could be used to accurately identify about 50 species of Aphidiinae that commonly occur in aphidparasitoid networks in northwestern Europe. We first identified species on a morphological basis and then assessed the consistency of genetic and morphological data. Probably because of mitochondrial introgression, Aphidius ervi and A.microlophii were indistinguishable on the basis of their COI sequences, whereas LWRh sequences discriminated these species. Conversely, because of its lower variability, LWRh failed to discriminate two pairs of species (Aphidius aquilus, Aphidius salicis, Lysiphlebus confusus and Lysiphlebus fabarum). Our study showed that no unique locus but a combination of two genes should be used to accurately identify members of Aphidiinae.

Dates et versions

hal-00849221 , version 1 (30-07-2013)

Identifiants

Citer

S.A.P. Derocles, Anne Le Ralec, Manuel Plantegenest, B. Chaubet, C. Cruaud, et al.. Identification of molecular markers for DNA barcoding in the Aphidiinae (Hym. Braconidae). Molecular Ecology Resources, 2012, 12 (2), pp.197-208. ⟨10.1111/j.1755-0998.2011.03083.x⟩. ⟨hal-00849221⟩
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