Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

BK/TD models for analyzing in vitro impedance data on cytotoxicity.

Abstract : The ban of animal testing has enhanced the development of new in vitro technologies for cosmetics safety assessment. Impedance metrics is one such technology which enables monitoring of cell viability in real time. However, analyzing real time data requires moving from static to dynamic toxicity assessment. In the present study, we built mechanistic biokinetic/toxicodynamic (BK/TD) models to analyze the time course of cell viability in cytotoxicity assay using impedance. These models account for the fate of the tested compounds during the assay. BK/TD models were applied to analyze HepaRG cell viability, after single (48h) and repeated (4 weeks) exposures to three hepatotoxic compounds (coumarin, isoeugenol and benzophenone-2). The BK/TD models properly fit the data used for their calibration that was obtained for single or repeated exposure. Only for one out of the three compounds, the models calibrated with a single exposure were able to predict repeated exposure data. We therefore recommend the use of long-term exposure in vitro data in order to adequately account for chronic hepatotoxic effects. The models we propose here are capable of being coupled with human biokinetic models in order to relate dose exposure and human hepatotoxicity.
Type de document :
Article dans une revue
Liste complète des métadonnées

Littérature citée [38 références]  Voir  Masquer  Télécharger

https://hal-agroparistech.archives-ouvertes.fr/hal-01146616
Déposant : Alexandre Pery <>
Soumis le : jeudi 28 mai 2020 - 10:46:00
Dernière modification le : jeudi 25 juin 2020 - 20:51:13

Fichier

2015_Teng_Toxicology Letters_1...
Fichiers éditeurs autorisés sur une archive ouverte

Licence


Distributed under a Creative Commons Paternité - Pas d'utilisation commerciale - Pas de modification 4.0 International License

Identifiants

Citation

S Teng, S Barcellini-Couget, R Beaudouin, C Brochot, G Desousa, et al.. BK/TD models for analyzing in vitro impedance data on cytotoxicity.. Toxicology Letters, Elsevier, 2015, 235 (2), pp.96-106. ⟨10.1016/j.toxlet.2015.03.011⟩. ⟨hal-01146616⟩

Partager

Métriques

Consultations de la notice

301

Téléchargements de fichiers

32