NOF1 encodes an arabidopsis protein involved in the control of rRNA expression
Résumé
The control of ribosomal RNA biogenesis is essential for the regulation of protein synthesis in eukaryotic cells. Here, we report the characterization of NOF1 that encodes a putative nucleolar protein involved in the control of rRNA expression in Arabidopsis. The gene has been isolated by T-DNA tagging and its function verified by the characterization of a second allele and genetic complementation of the mutants. The nof1 mutants are affected in female gametogenesis and embryo development. This result is consistent with the detection of NOF1 mRNA in all tissues throughout plant life's cycle, and preferentially in differentiating cells. Interestingly, the closely related proteins from zebra fish and yeast are also necessary for cell division and differentiation. We showed that the nof1-1 mutant displays higher rRNA expression and hypomethylation of rRNA promoter. Taken together, the results presented here demonstrated that NOF1 is an Arabidopsis gene involved in the control of rRNA expression, and suggested that it encodes a putative nucleolar protein, the function of which may be conserved in eukaryotes.
Mots clés
amino acid sequence
arabidopsis chemistry
arabidopsis genetics
arabidopsis metabolism
arabidopsis proteins chemistry
arabidopsis proteins genetics
arabidopsis proteins metabolism
cell nucleolus chemistry
cell nucleolus genetics
cell nucleolus metabolism
gene expression regulation plant
molecular sequence data
nuclear proteins chemistry
nuclear proteins genetics
nuclear proteins metabolism
protein transport
rna plant genetics
rna plant metabolism
rna ribosomal genetics
rna ribosomal metabolism
sequence alignment
génétique
cellule nucléole
expression de gène
séquence moléculaire
Domaines
Biologie cellulaire
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