Skip to Main content Skip to Navigation
New interface
Journal articles

Improved saccharification and ethanol yield from field-grown transgenic poplar deficient in cinnamoyl-CoA reductase

Abstract : Lignin is one of the main factors determining recalcitrance to enzymatic processing of lignocellulosic biomass. Poplars (Populus tremula x Populus alba) down-regulated for cinnamoyl-CoA reductase (CCR), the enzyme catalyzing the first step in the monolignol-specific branch of the lignin biosynthetic pathway, were grown in field trials in Belgium and France under short-rotation coppice culture. Wood samples were classified according to the intensity of the red xylem coloration typically associated with CCR down-regulation. Saccharification assays under different pretreatment conditions (none, two alkaline, and one acid pretreatment) and simultaneous saccharification and fermentation assays showed that wood from the most affected transgenic trees had up to 161% increased ethanol yield. Fermentations of combined material from the complete set of 20-mo-old CCR-down-regulated trees, including bark and less efficiently down-regulated trees, still yielded ∼ 20% more ethanol on a weight basis. However, strong down-regulation of CCR also affected biomass yield. We conclude that CCR down-regulation may become a successful strategy to improve biomass processing if the variability in down-regulation and the yield penalty can be overcome.
Document type :
Journal articles
Complete list of metadata

Cited literature [38 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01204007
Contributor : Archive Ouverte ProdInra Connect in order to contact the contributor
Submitted on : Wednesday, May 27, 2020 - 10:32:14 PM
Last modification on : Friday, August 5, 2022 - 2:38:10 PM

File

PNAS-2014-Van Acker-845-50_1.p...
Publisher files allowed on an open archive

Identifiers

Citation

Rebecca van Acker, Jean-Charles Leplé, Dirk Aerts, Véronique Storme, Geert Goeminne, et al.. Improved saccharification and ethanol yield from field-grown transgenic poplar deficient in cinnamoyl-CoA reductase. Proceedings of the National Academy of Sciences of the United States of America, 2014, 111 (2), pp.845-850. ⟨10.1073/pnas.1321673111⟩. ⟨hal-01204007⟩

Share

Metrics

Record views

70

Files downloads

32