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Using a physiological framework for improving the detection of quantitative trait loci related to nitrogen nutrition in Medicago truncatula

Abstract : [i]Medicago truncatula[/i] is used as a model plant for exploring the genetic and molecular determinants of nitrogen (N) nutrition in legumes. In this study, our aim was to detect quantitative trait loci (QTL) controlling plant N nutrition using a simple framework of carbon/N plant functioning stemming from crop physiology. This framework was based on efficiency variables which delineated the plant's efficiency to take up and process carbon and N resources. A recombinant inbred line population (LR4) was grown in a glasshouse experiment under two contrasting nitrate concentrations. At low nitrate, symbiotic N-2 fixation was the main N source for plant growth and a QTL with a large effect located on linkage group (LG) 8 affected all the traits. Significantly, efficiency variables were necessary both to precisely localize a second QTL on LG5 and to detect a third QTL involved in epistatic interactions on LG2. At high nitrate, nitrate assimilation was the main N source and a larger number of QTL with weaker effects were identified compared to low nitrate. Only two QTL were common to both nitrate treatments: a QTL of belowground biomass located at the bottom of LG3 and another one on LG6 related to three different variables (leaf area, specific N uptake and aboveground:belowground biomass ratio). Possible functions of several candidate genes underlying QTL of efficiency variables could be proposed. Altogether, our results provided new insights into the genetic control of N nutrition in M. truncatula. For instance, a novel result for [i]M. truncatula[/i] was identification of two epistatic interactions in controlling plant N-2 fixation. As such this study showed the value of a simple conceptual framework based on efficiency variables for studying genetic determinants of complex traits and particularly epistatic interactions
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https://hal.archives-ouvertes.fr/hal-01267800
Déposant : Archive Ouverte Prodinra <>
Soumis le : jeudi 4 février 2016 - 20:26:32
Dernière modification le : jeudi 15 octobre 2020 - 03:17:34

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Delphine Moreau, Judith Burstin, Gregoire Aubert, Thierry Huguet, Cécile Ben, et al.. Using a physiological framework for improving the detection of quantitative trait loci related to nitrogen nutrition in Medicago truncatula. TAG Theoretical and Applied Genetics, Springer Verlag, 2012, 124 (4), pp.755-768. ⟨10.1007/s00122-011-1744-z⟩. ⟨hal-01267800⟩

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