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Gut Commensal E. coli Proteins Activate Host Satiety Pathways following Nutrient-Induced Bacterial Growth

Jonathan Breton 1, 2 Naouel Tennoune 2, 1 Luc Lucas 2, 1 Marie Francois 2, 1 Romain Legrand 2, 1 Justine Jacquemot 2, 1 Alexis Goichon 2 Charlène Guérin 2, 1 Johann Peltier 3, 1 Martine Pestel-Caron 4, 3 Philippe Chan 4, 1 David Vaudry 4, 1 Jean-Claude Do rego 5, 1 Fabienne Liénard 6 Luc Pénicaud 6 Xavier Fioramonti 6 Ivor s. Ebenezer 7 Tomas Hökfelt 8 Pierre Déchelotte 2, 4, 5 Sergueï o. Fetissov 2, 1, *
* Auteur correspondant
1 IRIB - Institute for Research and Innovation in Biomedicine
2 ADEN - Nutrition, inflammation et dysfonctionnement de l'axe intestin-cerveau
3 GRAM 1.0 - Groupe de Recherche sur les Antimicrobiens et les Micro-Organismes
4 UNIROUEN - Université de Rouen Normandie
5 Hôpital Charles Nicolle [Tunis]
6 CSGA - Centre des Sciences du Goût et de l'Alimentation [Dijon]
7 University of Portsmouth
8 Karolinska Institutet [Stockholm]
Abstract : The composition of gut microbiota has been associated with host metabolic phenotypes, but it is not known if gut bacteria may influence host appetite. Here we show that regular nutrient provision stabilizes exponential growth of E. coli, with the stationary phase occurring 20 min after nutrient supply accompanied by bacterial proteome changes, suggesting involvement of bacterial proteins in host satiety. Indeed, intestinal infusions of E. coli stationary phase proteins increased plasma PYY and their intraperitoneal injections suppressed acutely food intake and activated c-Fos in hypothalamic POMC neurons, while their repeated administrations reduced meal size. ClpB, a bacterial protein mimetic of alpha-MSH, was upregulated in the E. coli stationary phase, was detected in plasma proportional to ClpB DNA in feces, and stimulated firing rate of hypothalamic POMC neurons. Thus, these data show that bacterial proteins produced after nutrient-induced E. coli growth may signal meal termination. Furthermore, continuous exposure to E. coli proteins may influence long-term meal pattern.
Keywords : neuropeptide-y arcuate nucleus escherichia-coli glucagon-like peptide-1 melanocortin-4 receptor intestinal microbiota energy homeostasis food-intake high-fat in-vivo
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Article dans une revue
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https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01397996
Déposant : Csga - Université de Bourgogne <>
Soumis le : mercredi 16 novembre 2016 - 15:19:02
Dernière modification le : mercredi 1 juillet 2020 - 13:24:02

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UNIV-BOURGOGNE | CNRS | INRA | COMUE-NORMANDIE | CSGA | UNIROUEN | GRAM | INRAE

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Jonathan Breton, Naouel Tennoune, Luc Lucas, Marie Francois, Romain Legrand, et al.. Gut Commensal E. coli Proteins Activate Host Satiety Pathways following Nutrient-Induced Bacterial Growth. Cell Metabolism, Elsevier, 2016, 23 (2), pp.324 - 334. ⟨10.1016/j.cmet.2015.10.017⟩. ⟨hal-01397996⟩

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