Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

New Insight into Daptomycin Bioavailability and Localization in Staphylococcus aureus Biofilms by Dynamic Fluorescence Imaging

Abstract : Staphylococcus aureus is one of the most frequent pathogens responsible for biofilm-associated infections (BAI), and the choice of antibiotics to treat these infections remains a challenge for the medical community. In particular, daptomycin has been reported to fail against implant-associated S. aureus infections in clinical practice, while its association with rifampin remains a good candidate for BAI treatment. To improve our understanding of such resistance/tolerance toward daptomycin, we took advantage of the dynamic fluorescence imaging tools (time-lapse imaging and fluorescence recovery after photobleaching [FRAP]) to locally and accurately assess the antibiotic diffusion reaction in methicillin-susceptible and methicillin-resistant S. aureus biofilms. To provide a realistic representation of daptomycin action, we optimized an in vitro model built on the basis of our recently published in vivo mouse model of prosthetic vascular graft infections. We demonstrated that at therapeutic concentrations, daptomycin was inefficient in eradicating biofilms, while the matrix was not a shield to antibiotic diffusion and to its interaction with its bacterial target. In the presence of rifampin, daptomycin was still present in the vicinity of the bacterial cells, allowing prevention of the emergence of rifampin-resistant mutants. Conclusions derived from this study strongly suggest that S. aureus biofilm resistance/tolerance toward daptomycin may be more likely to be related to a physiological change involving structural modifications of the membrane, which is a strain-dependent process.
Type de document :
Article dans une revue
Liste complète des métadonnées
Déposant : Laurent Jonchère <>
Soumis le : lundi 6 mars 2017 - 15:36:31
Dernière modification le : vendredi 20 novembre 2020 - 10:38:09
Archivage à long terme le : : mercredi 7 juin 2017 - 14:57:27



Rym Boudjemaa, Romain Briandet, Matthieu Revest, Cédric Jacqueline, Jocelyne Caillon, et al.. New Insight into Daptomycin Bioavailability and Localization in Staphylococcus aureus Biofilms by Dynamic Fluorescence Imaging. Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 2016, 60 (8), pp.4983-4990. ⟨10.1128/AAC.00735-16⟩. ⟨hal-01435012⟩



Consultations de la notice


Téléchargements de fichiers