HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Hydrosol of Thymbra capitata Is a Highly Efficient Biocide against Salmonella enterica Serovar Typhimurium Biofilms

Abstract : Salmonella is recognized as one of the most significant enteric foodborne bacterial pathogens. In recent years, the resistance of pathogens to biocides and other environmental stresses, especially when they are embedded in biofilm structures, has led to the search for and development of novel antimicrobial strategies capable of displaying both high efficiency and safety. In this direction, the aims of the present work were to evaluate the antimicrobial activity of hydrosol of the Mediterranean spice Thymbra capitata against both planktonic and biofilm cells of Salmonella enterica serovar Typhimurium and to compare its action with that of benzalkonium chloride (BC), a commonly used industrial biocide. In order to achieve this, the disinfectant activity following 6-min treatments was comparatively evaluated for both disinfectants by calculating the concentrations needed to achieve the same log reductions against both types of cells. Their bactericidal effect against biofilm cells was also comparatively determined by in situ and real-time visualization of cell inactivation through the use of time-lapse confocal laser scanning microscopy (CLSM). Interestingly, results revealed that hydrosol was almost equally effective against biofilms and planktonic cells, whereas a 200-times-higher concentration of BC was needed to achieve the same effect against biofilm compared to planktonic cells. Similarly, time-lapse CLSM revealed the significant advantage of the hydrosol to easily penetrate within the biofilm structure and quickly kill the cells, despite the three-dimensional (3D) structure of Salmonella biofilm.
Complete list of metadata

https://hal-agroparistech.archives-ouvertes.fr/hal-01569015
Contributor : Armelle Sielinou Connect in order to contact the contributor
Submitted on : Thursday, April 21, 2022 - 12:13:42 AM
Last modification on : Thursday, April 21, 2022 - 8:44:27 AM

File

Karampoula text V2_2016June09_...
Files produced by the author(s)

Identifiers

Citation

F. Karampoula, E. Giaouris, J. Deschamps, A.I. Dougleraki, G.-J.E. Nychas, et al.. Hydrosol of Thymbra capitata Is a Highly Efficient Biocide against Salmonella enterica Serovar Typhimurium Biofilms. Applied and Environmental Microbiology, American Society for Microbiology, 2016, 82 (17), pp.5309-5319. ⟨10.1128/AEM.01351-16⟩. ⟨hal-01569015⟩

Share

Metrics

Record views

105

Files downloads

6