Skip to Main content Skip to Navigation
Journal articles

Estimating virus effective population size and selection without neutral markers

Abstract : By combining high-throughput sequencing (HTS) with experimental evolution, we can observe the within-host dynamics of pathogen variants of biomedical or ecological interest. We studied the evolutionary dynamics of five variants of Potato virus Y (PVY) in 15 doubled-haploid lines of pepper. All plants were inoculated with the same mixture of virus variants and, variant frequencies were determined by HTS in eight plants of each pepper line, at each of six sampling dates. We developed a method for estimating the intensities of selection and genetic drift in a multi-allelic Wright-Fisher model, applicable whether these forces are strong or weak, and in the absence of neutral markers. This method requires variant frequency determination at several time points, in independent hosts. The parameters are the selection coefficients for each PVY variant and four effective population sizes N e at different time-points of the experiment. Numerical simulations of asexual haploid Wright-Fisher populations subjected to contrasting genetic drift (N e 2 [10, 2000]) and selection (|s| 2 [0, 0.15]) regimes were used to validate the method proposed. The experiment in closely related pepper host genotypes revealed that viruses experienced a considerable diversity of selection and genetic drift regimes. The resulting variant dynamics were accurately described by Wright-Fisher models. The fitness ranks of the variants were almost identical between host genotypes. By contrast, the dynamics of N e were highly variable, although a bottleneck was often identified during the systemic movement of the virus. We demonstrated that, for a fixed initial PVY population, virus effective population size is a heritable trait in plants. These findings pave the way for the breeding of plant varieties exposing viruses to stronger genetic drift, thereby slowing virus adaptation. PLOS Pathogens |
Complete list of metadata

Cited literature [60 references]  Display  Hide  Download
Contributor : Jean-Luc Gouzé <>
Submitted on : Monday, December 11, 2017 - 5:57:15 PM
Last modification on : Tuesday, July 13, 2021 - 3:23:10 AM


Publisher files allowed on an open archive


Distributed under a Creative Commons Attribution 4.0 International License



Elsa Rousseau, Benoit Moury, Ludovic Mailleret, Rachid Senoussi, Alain Palloix, et al.. Estimating virus effective population size and selection without neutral markers. PLoS Pathogens, Public Library of Science, 2017, 13 (11), pp.1-25. ⟨10.1371/journal.ppat.1006702⟩. ⟨hal-01658535v2⟩



Record views


Files downloads