PEI-cellulose nanocrystal hybrids as efficient siRNA delivery agents—Synthesis, physicochemical characterization and in vitro evaluation - Archive ouverte HAL Access content directly
Journal Articles Carbohydrate Polymers Year : 2017

PEI-cellulose nanocrystal hybrids as efficient siRNA delivery agents—Synthesis, physicochemical characterization and in vitro evaluation

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Abstract

We describe the synthesis of cellulose nanocrystals (CNCs) complexed to siRNA. PEI (600 Da) was first covalently attached to CNCs by reductive amination reaction, then CNCs-PEI were loaded with siRNA, resulting in the formation of CNCs-PEI-siRNA particles, strongly stabilized by ionic interactions. Efficient cellular uptake of these particles was monitored by ethidium bromide staining. Not only did CNCs-PEI show no cytotoxicity at the studied concentrations, but they also protected siRNA from degradation and favored its delivery into the cells. This siRNA (siRNA killer) is able to silence the expression of cell cycle genes and to induce cell death by apoptosis. Therefore, this study suggests that these CNCs-PEI are promising non-viral nanovehicles for siRNA delivery and for efficient anti-tumor strategy.
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Dates and versions

hal-01738117 , version 1 (20-03-2018)

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Gautier Mark Arthur Ndong Ntoutoume, Vincent Grassot, Frédérique Brégier, Julien Chabanais, Jean-Michel Petit, et al.. PEI-cellulose nanocrystal hybrids as efficient siRNA delivery agents—Synthesis, physicochemical characterization and in vitro evaluation. Carbohydrate Polymers, 2017, 164, pp.258 - 267. ⟨10.1016/j.carbpol.2017.02.004⟩. ⟨hal-01738117⟩
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