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Isolation and characterization of microsatellites in the endoparasitic ichneumonid wasp carrying a polydnavirus Hyposoter didymator

Abstract : The wasp Hyposoter didymator (Hymenoptera, Ichneumonidae) parasitizes several agricultural pest moths and could therefore be used in biological control. The 454 FLX Titanium pyrosequencing technology was used to define two distinct sets of multiplex combining 14 polymorphic microsatellite loci: 10 (referred to as HD) located within the genome of H. didymator and 4 (referred to as HdIV) located within the Ichnovirus genome which is integrated into the wasp genome. Genotyping of two populations collected in France on Helicoverpa armigera revealed that most of the loci are independent and at Hardy-Weinberg equilibrium.
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Philippe Audiot, Véronique Jouan, Marie Frayssinet, Anne-Nathalie Volkoff, Denis Bourguet. Isolation and characterization of microsatellites in the endoparasitic ichneumonid wasp carrying a polydnavirus Hyposoter didymator. Conservation Genetics Resources, Springer, 2014, 6 (1), pp.21-23. ⟨10.1007/s12686-013-0036-0⟩. ⟨hal-01837256⟩

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