Skip to Main content Skip to Navigation
Journal articles

Comparative genomics between two Xenorhabdus bovienii strains highlights differential evolutionary scenarios within an entomopathogenic bacterial species

Abstract : Bacteria of the genus Xenorhabdus are symbionts of soil entomopathogenic nematodes of the genus Steinernema. This symbiotic association constitutes an insecticidal complex active against a wide range of insect pests. Within Xenorhabdus bovienii species, the X. bovienii CS03 strain (Xb CS03) is nonvirulent when directly injected into lepidopteran insects, and displays a low virulence when associated with its Steinernema symbiont. The genome of Xb CS03 was sequenced and compared with the genome of a virulent strain, X. bovienii SS-2004 (Xb SS-2004). The genome size and content widely differed between the twostrains. Indeed,XbCS03had a large genomecontaining several specific loci involved in the inhibition of competitors, including a fewNRPS-PKS loci (nonribosomal peptide synthetases and polyketide synthases) producing antimicrobialmolecules. Consistently, Xb CS03 had a greater antimicrobial activity than Xb SS-2004. The Xb CS03 strain contained more pseudogenes than Xb SS-2004. Decay of genes involved in the host invasion and exploitation (toxins, invasins, or extracellular enzymes) was particularly important in Xb CS03. This may provide an explanation for the nonvirulence of the strain when injected into an insect host.We suggest that Xb CS03 and Xb SS-2004 followed divergent evolutionary scenarios to cope with their peculiar life cycle. The fitness strategy of Xb CS03 would involve competitor inhibition, whereas Xb SS-2004 would quickly and efficiently kill the insect host. Hence, Xenorhabdus strains would have widely divergent host exploitation strategies, which impact their genome structure.
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-01837293
Contributor : Archive Ouverte ProdInra Connect in order to contact the contributor
Submitted on : Wednesday, May 27, 2020 - 6:21:35 PM
Last modification on : Friday, August 5, 2022 - 10:44:24 AM

File

2016_Bisch_Genome Biology and ...
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Gaelle Bisch, Jean-Claude Ogier, Claudine Médigue, Zoé Rouy, Stéphanie Vincent, et al.. Comparative genomics between two Xenorhabdus bovienii strains highlights differential evolutionary scenarios within an entomopathogenic bacterial species. Genome Biology and Evolution, Society for Molecular Biology and Evolution, 2016, 8 (1), pp.148-160. ⟨10.1093/gbe/evv248⟩. ⟨hal-01837293⟩

Share

Metrics

Record views

70

Files downloads

30