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The gp27-like Hub of VgrG Serves as Adaptor to Promote Hcp Tube Assembly

Abstract : Contractile injection systems are multiprotein complexes that use a spring-like mechanism to deliver effectors into target cells. In addition to using a conserved mechanism, these complexes share a common core known as the tail. The tail comprises an inner tube tipped by a spike, wrapped by a contractile sheath, and assembled onto a baseplate. Here, using the type VI secretion system (T6SS) as a model of contractile injection systems, we provide molecular details on the interaction between the inner tube and the spike. Reconstitution into the Escherichia coli heterologous host in the absence of other T6SS components and in vitro experiments demonstrated that the Hcp tube component and the VgrG spike interact directly. VgrG deletion studies coupled to functional assays showed that the N-terminal domain of VgrG is sufficient to interact with Hcp, to initiate proper Hcp tube polymerization, and to promote sheath dynamics and Hcp release. The interaction interface between Hcp and VgrG was then mapped using docking simulations, mutagenesis, and cysteine-mediated cross-links. Based on these results, we propose a model in which the VgrG base serves as adaptor to recruit the first Hcp hexamer and initiates inner tube polymerization.
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Déposant : Eric Cascales <>
Soumis le : lundi 23 juillet 2018 - 11:55:14
Dernière modification le : dimanche 31 mai 2020 - 07:54:01
Archivage à long terme le : : mercredi 24 octobre 2018 - 14:23:01


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Melvin Renault, Jordi Zamarreño Beas, Badreddine Douzi, Maïalène Chabalier, Abdelrahim Zoued, et al.. The gp27-like Hub of VgrG Serves as Adaptor to Promote Hcp Tube Assembly. Journal of Molecular Biology, Elsevier, 2018, 430 (18 Pt B), pp.3143-3156. ⟨10.1016/j.jmb.2018.07.018⟩. ⟨hal-01847145⟩



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