Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

The complete methylome of an entomopathogenic bacterium reveals the existence of loci with unmethylated Adenines

Abstract : DNA methylation can serve to control diverse phenomena in eukaryotes and prokaryotes, including gene regulation leading to cell differentiation. In bacteria, DNA methylomes (i.e., methylation state of each base of the whole genome) have been described for several species, but methylome profile variation during the lifecycle has rarely been studied, and only in a few model organisms. Moreover, major phenotypic changes have been reported in several bacterial strains with a deregulated methyltransferase, but the corresponding methylome has rarely been described. Here we report the first methylome description of an entomopathogenic bacterium, Photorhabdus luminescens. Eight motifs displaying a high rate of methylation (> 94%) were identified. The methylome was strikingly stable over course of growth, but also in a subpopulation responsible for a critical step in the bacterium's lifecycle: successful survival and proliferation in insects. The rare unmethylated GATC motifs were preferentially located in putative promoter regions, and most of them were methylated after Dam methyltransferase overexpression, suggesting that DNA methylation is involved in gene regulation. Our findings bring key insight into bacterial methylomes and encourage further research to decipher the role of loci protected from DNA methylation in gene regulation.
Liste complète des métadonnées

Littérature citée [70 références]  Voir  Masquer  Télécharger

https://hal.archives-ouvertes.fr/hal-01867979
Déposant : Archive Ouverte Prodinra <>
Soumis le : mardi 4 septembre 2018 - 20:05:45
Dernière modification le : jeudi 4 février 2021 - 11:48:02

Fichier

publi18-lipm-020_playelleville...
Accord explicite pour ce dépôt

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Collections

Citation

Amaury Payelleville, Ludovic Legrand, Jean-Claude Ogier, Cécile Roques, Alain Roulet, et al.. The complete methylome of an entomopathogenic bacterium reveals the existence of loci with unmethylated Adenines. Scientific Reports, Nature Publishing Group, 2018, 8 (1), pp.1-14. ⟨10.1038/s41598-018-30620-5⟩. ⟨hal-01867979⟩

Partager

Métriques

Consultations de la notice

1040

Téléchargements de fichiers

188