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Identification by Tn-seq of Dickeya dadantii genes required for survival in chicory plants

Abstract : he identification of the virulence factors of plant‐pathogenic bacteria has relied on the testing of individual mutants on plants, a time‐consuming process. Transposon sequencing (Tn‐seq) is a very powerful method for the identification of the genes required for bacterial growth in their host. We used this method in a soft‐rot pathogenic bacterium to identify the genes required for the multiplication of Dickeya dadantii in chicory. About 100 genes were identified showing decreased or increased fitness in the plant. Most had no previously attributed role in plant–bacterium interactions. Following our screening, in planta competition assays confirmed that the uridine monophosphate biosynthesis pathway and the purine biosynthesis pathway were essential to the survival of D. dadantii in the plant, as the mutants ∆carA, ∆purF, ∆purL, ∆guaB and ∆pyrE were unable to survive in the plant in contrast with the wild‐type (WT) bacterium. This study also demonstrated that the biosynthetic pathways of leucine, cysteine and lysine were essential for bacterial survival in the plant and that RsmC and GcpA were important in the regulation of the infection process, as the mutants ∆rsmC and ∆gcpA were hypervirulent. Finally, our study showed that D. dadantii flagellin was glycosylated and that this modification conferred fitness to the bacterium during plant infection. Assay by this method of the large collections of environmental pathogenic strains now available will allow an easy and rapid identification of new virulence factors.
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https://hal.archives-ouvertes.fr/hal-01922287
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Soumis le : mercredi 14 novembre 2018 - 14:17:18
Dernière modification le : vendredi 25 septembre 2020 - 09:52:02
Archivage à long terme le : : vendredi 15 février 2019 - 14:36:07

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Kévin Royet, Nicolas Parisot, Agnès Rodrigue, Erwan Gueguen, Guy Condemine. Identification by Tn-seq of Dickeya dadantii genes required for survival in chicory plants. Molecular Plant Pathology, Wiley, 2019, 20 (2), pp.287-306. ⟨10.1111/mpp.12754⟩. ⟨hal-01922287⟩

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